Difference between revisions of "Part:BBa K1349006:Design"
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| − | + | Amplified by PCR from E.coli W3110 genomic DNA. | |
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===References=== | ===References=== | ||
Latest revision as of 07:48, 9 October 2014
cheA
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1636
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is the CheA histidine Kinase that forms part of the Chemotaxis system in E. coli. Naturally this protein is able to auto-phosphorylate and transfer the phosphate groups to CheB and CheY.
Obtained by PCR from E.coli and SLIC assembly. The construction removed the EcoR1 restriction site in the gene by silent mutations of ATT codon to a ATC codon. the construction contains the entire coding sequence.
Expression of this part is expected to complement the chemotaxis minus phenotype of a CheA mutant.
Source
Amplified by PCR from E.coli W3110 genomic DNA.