Difference between revisions of "Part:BBa K1349005:Design"

(→‎Design Notes)
(→‎References)
 
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===References===
 
===References===
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Whitaker WR, Davis SA, Arkin AP, Dueber JE. 2012.
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Engineering robust control of two-component system phosphotransfer using modular scaffolds.
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''Proc Natl Acad Sci U S A.'' '''109(44):'''18090-5. doi: 10.1073/pnas.1209230109.

Latest revision as of 07:43, 9 October 2014


SH3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 211
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 136


Design Notes

This part is a protein module designed as part of a synthetic protein-protein interaction system developed by Whitaker et al. The small SH3 domain should recognize and bind the SH3peptide in part BBa_K1349008

A fusion protein containing this part is expected to interact/bind with the part BBa_K1349008.

Source

Amplified from pWW2179, Whitaker et al. PNAS 2012

References

Whitaker WR, Davis SA, Arkin AP, Dueber JE. 2012. Engineering robust control of two-component system phosphotransfer using modular scaffolds. Proc Natl Acad Sci U S A. 109(44):18090-5. doi: 10.1073/pnas.1209230109.