Difference between revisions of "Part:BBa K1349001:Design"

(→‎Design Notes)
(→‎Source)
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===Source===
 
===Source===
  
−
The relA gene has been amplified from E. coli W3110 genomic DNA.
+
The part was obtained by PCR from the pT18 RelA provided by Emmanuelle Bouveret and SLIC assembly. The construction removed the PstI restriction site in the gene by the silent mutation of a CTG codon to a CTC codon. The construction contains the entire coding sequence.
  
 
===References===
 
===References===

Revision as of 07:22, 9 October 2014


relA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1708


Design Notes

This part is designed to allow the rapid synthesis of the alarmone ppGpp, responsible for the stringent response in E. coli. In the context of our project ppGpp is accumulation used to stop initiation of cell division. This part is complementary to part BBa_K1349004.

Expression is expected to cause accumulation of ppGpp and so reduce growth rate. Expression in a mutant unable to make ppGpp is expected to complement the mutant. This complementation has been tested and is observed.

Source

The part was obtained by PCR from the pT18 RelA provided by Emmanuelle Bouveret and SLIC assembly. The construction removed the PstI restriction site in the gene by the silent mutation of a CTG codon to a CTC codon. The construction contains the entire coding sequence.

References