Difference between revisions of "Part:BBa K1465229:Design"
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===Design Notes=== | ===Design Notes=== | ||
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+ | Classical [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#BioBrick Suffix Insertion] of the Sedoheptulose-1,7-Bisphosphatase (''glpX'') (<bbpart>BBa_K1465228</bbpart>) into the pSB1C3 backbone of the P<sub>tac</sub> promotor containing also the constitutive lacI repressor (<bbpart>BBa_K731500</bbpart>). | ||
===Source=== | ===Source=== | ||
− | + | <bbpart>BBa_K1465228</bbpart>: Sedoheptulose-1,7-Biphosphatase (''glpX'') from ''Bacillus methanolicus''<br> | |
+ | <bbpart>BBa_K731500</bbpart>: [LacI + LacIq promoter] reverse + [tac promoter + lac operator] forward | ||
===References=== | ===References=== |
Latest revision as of 22:46, 8 October 2014
Sedoheptulose 1,7-bisphosphatase (glpX) from Bacillus methanolicus under the control of ptac
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Classical [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#BioBrick Suffix Insertion] of the Sedoheptulose-1,7-Bisphosphatase (glpX) (BBa_K1465228) into the pSB1C3 backbone of the Ptac promotor containing also the constitutive lacI repressor (BBa_K731500).
Source
BBa_K1465228: Sedoheptulose-1,7-Biphosphatase (glpX) from Bacillus methanolicus
BBa_K731500: [LacI + LacIq promoter] reverse + [tac promoter + lac operator] forward