Difference between revisions of "Part:BBa K1510233:Design"

 
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===References===
 
===References===
 +
Bahassi EM1, O'Dea MH, Allali N, Messens J, Gellert M, Couturier M.( 1999 Apr 16) Interactions of CcdB with DNA gyrase. Inactivation of Gyra, poisoning of the gyrase-DNA complex, and the antidote action of CcdA

Revision as of 19:56, 8 October 2014


A blue light regulated ccdb apoptosis gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 472


Design Notes

Whether the blue light promoter is strong enough to turn on enough ccdb gene, and whether ccdb efficiency greater than cell division ability.


Source

The blue light promoter was derived from igem kit : K592006. And the ccdb gene was K145151.

References

Bahassi EM1, O'Dea MH, Allali N, Messens J, Gellert M, Couturier M.( 1999 Apr 16) Interactions of CcdB with DNA gyrase. Inactivation of Gyra, poisoning of the gyrase-DNA complex, and the antidote action of CcdA