Difference between revisions of "Part:BBa K1362100"

Revision as of 15:23, 8 October 2014

RBS + NpuDnaE N-intein RFC[105] assembly construct (with His6)

The OneGate assembly construct is part of the iGEM team Heidelberg 2014's strategy for cloning with split inteins. Inteins are naturally occuring peptide sequences that splice out of a precursor protein and attach the remaining ends together to form a new protein. When splitting those intein sequence into an N-terminal and a C-terminal split intein one is left with a powerful tool to post-translationally modify whole proteins on the amino-acid sequence level. This construct was designed to express any protein of interest fused to the Nostoc punctiforme DnaE N-terminal split intein.

Nostoc punctiforme DnaE split intein (NpuDnaE)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 826
Illegal AgeI site found at 938
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1115
Illegal BsaI.rc site found at 40

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 __NOTOC__
 <partinfo>BBa_K1362100 short</partinfo>
-???
+The OneGate assembly construct is part of the iGEM team Heidelberg 2014's strategy for cloning with split inteins. Inteins are naturally occuring peptide sequences that splice out of a precursor protein and attach the remaining ends together to form a new protein. When splitting those intein sequence into an N-terminal and a C-terminal split intein one is left with a powerful tool to post-translationally modify whole proteins on the amino-acid sequence level. This construct was designed to express any protein of interest fused to the <i>Nostoc punctiforme</i> DnaE N-terminal split intein.
-<!-- Add more about the biology of this part here
+===<i>Nostoc punctiforme</i> DnaE split intein (NpuDnaE)===
-===Usage and Biology===
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