Difference between revisions of "Part:BBa K1455003"

 
 
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<partinfo>BBa_K1455003 short</partinfo>
 
<partinfo>BBa_K1455003 short</partinfo>
  
luxOD47E gene followed by GFP reporter.  
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mRFP chromoprotein under tetracylcine-repressive promoter.
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Bacteria with this device are red due to the mRFP constitutive expression. This espression turns off if TetR repressor is present and it can be restored by the addition of tetracycline or analogues (as anhydrotetracycline).
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Here is an example of E. coli DH5α carrying this device:
  
 
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Latest revision as of 13:24, 8 October 2014

pTet - mRFP

mRFP chromoprotein under tetracylcine-repressive promoter. Bacteria with this device are red due to the mRFP constitutive expression. This espression turns off if TetR repressor is present and it can be restored by the addition of tetracycline or analogues (as anhydrotetracycline).

Here is an example of E. coli DH5α carrying this device:

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 635
    Illegal AgeI site found at 747
  • 1000
    COMPATIBLE WITH RFC[1000]