Difference between revisions of "Part:BBa K1455003"

Revision as of 02:37, 8 October 2014

pTet - mRFP

luxO D47E gene followed by GFP reporter.

This device can be assembled with any promoter to obatin any kind of expression for LusOD 47E and to measure it by fluorescence of GFP as was made in BBa_K1455002.

LuxOD 47E acts like a phosphorilated LuxO, which is an essential protein of Vibrio spp. quorum sensing system. LuxO is the final protein in a phophorilation cascade and binds, as a trancription facotr, to pqrr promoters. LuxOD 47E is able to activate the genes downstream of a pqrr promoter.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 635
Illegal AgeI site found at 747
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1455003 short</partinfo>
-luxOD47E gene followed by GFP reporter.
+luxO D47E gene followed by GFP reporter.
+This device can be assembled with any promoter to obatin any kind of expression for LusOD 47E and to measure it by fluorescence of GFP as was made in [https://parts.igem.org/Part:BBa_K1455002 BBa_K1455002].
+LuxOD 47E acts like a phosphorilated LuxO, which is an essential protein of Vibrio spp. quorum sensing system. LuxO is the final protein in a phophorilation cascade and binds, as a trancription facotr, to pqrr promoters. LuxOD 47E is able to activate the genes downstream of a pqrr promoter.
 <!-- Add more about the biology of this part here