Difference between revisions of "Part:BBa K1412006"
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<partinfo>BBa_K1412006 short</partinfo> | <partinfo>BBa_K1412006 short</partinfo> | ||
| − | This part is a derivative of [https://parts.igem.org/Part:BBa_K1412005 BBa_K1412005 ] but weaker than it. It consists of a CheZ coding region and a RBS coding region whose relative efficiency is 0. | + | __NOTOC__ |
| + | <partinfo>BBa_K1412006 short</partinfo> | ||
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| + | This part is a derivative of [https://parts.igem.org/Part:BBa_K1412005 BBa_K1412005 ] but weaker than it. It consists of a CheZ coding region and a RBS coding region whose relative efficiency is 0.3. This part does not pose any biological threat. With a promoter of your interest, this device rescues the mobility of CheZ-/- cells. It has been reported that CheZ-/- strain has a higher frequency of direction change and thus a narrower range of mobility. | ||
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[1] [http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0011915#pone-0011915-g006 Paungfoo-Lonhienne C et al. (2010) Turning the table: plants consume microbes as a source of nutrients. PLoS One 5(7): e11915 ]. | [1] [http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0011915#pone-0011915-g006 Paungfoo-Lonhienne C et al. (2010) Turning the table: plants consume microbes as a source of nutrients. PLoS One 5(7): e11915 ]. | ||
| − | [2] [http://pubs.acs.org/doi/abs/10.1021/bi00561a015 Chelsky D and Dahlquist FW (1980) Chemotaxis in Escherichia | + | [2] [http://pubs.acs.org/doi/abs/10.1021/bi00561a015 Chelsky D and Dahlquist FW (1980) Chemotaxis in Escherichia |
Revision as of 15:09, 7 October 2014
RBS (0.01) CheZ double terminators -- derivative of BBa_K1412005
RBS (0.01) CheZ double terminators -- derivative of BBa_K1412005
This part is a derivative of BBa_K1412005 but weaker than it. It consists of a CheZ coding region and a RBS coding region whose relative efficiency is 0.3. This part does not pose any biological threat. With a promoter of your interest, this device rescues the mobility of CheZ-/- cells. It has been reported that CheZ-/- strain has a higher frequency of direction change and thus a narrower range of mobility.
Usage
1.We performed a swarming assay to characterize the motility of CheZ-deficient strain CL1 and cells transformed with CheZ-expressing plasmid BBa_K1412001 .
Our results clearly show that CheZ-deficient mutants show smaller diffusion, while they are rescued by our BBa_K1412001 plasmid.
2.We also performed a swarming assay to characterize the strength of RBS with CL1 cells. Three strains of CL1 are transformed with CheZ-expressing plasmid of different RBS efficiency (BBa_B0034 :1.0 ; BBa_B0032 :0.3 ; BBa_B0033 :0.01 ).
Changing different RBS in the same circuit, we can characterize the efficiency of RBS, since the expression strength of CheZ is positively associated with the efficiency of RBS.
Experimental data
;
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Reference
[1] [http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0011915#pone-0011915-g006 Paungfoo-Lonhienne C et al. (2010) Turning the table: plants consume microbes as a source of nutrients. PLoS One 5(7): e11915 ].
[2] [http://pubs.acs.org/doi/abs/10.1021/bi00561a015 Chelsky D and Dahlquist FW (1980) Chemotaxis in Escherichia