Difference between revisions of "Part:BBa K1351024"
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<partinfo>BBa_K1351024 short</partinfo> | <partinfo>BBa_K1351024 short</partinfo> | ||
− | CSP-depending promoter of ''Streptococcus pneumoniae'' (R6). This regulatory | + | CSP-depending promoter of ''Streptococcus pneumoniae'' (R6). This regulatory site has been shown to be sufficient to drive ComE regulated transcription within ''S. pneumoniae''[http://www.ncbi.nlm.nih.gov/pubmed/?term=comE%2FcomE~P+interplay]. |
This part was generated with the RFC10 standard and has the following prefix and suffix: | This part was generated with the RFC10 standard and has the following prefix and suffix: |
Revision as of 12:21, 7 October 2014
PcomC, a CSP inducible promoter derived from Streptococcus pneumoniae
CSP-depending promoter of Streptococcus pneumoniae (R6). This regulatory site has been shown to be sufficient to drive ComE regulated transcription within S. pneumoniae[http://www.ncbi.nlm.nih.gov/pubmed/?term=comE%2FcomE~P+interplay].
This part was generated with the RFC10 standard and has the following prefix and suffix:
prefix with EcoRI, NotI and XbaI: | GAATTCGCGGCCGCTTCTAGAG |
suffix with SpeI, NotI and PstI: | TACTAGTAGCGGCCGCTGCAG |
Sites of restriction enzymes generating compatible overhangs have the same color:
EcoRI and PstI in blue, NotI in green, XbaI and SpeI in red.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]