Difference between revisions of "Part:BBa K1379007:Design"
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<partinfo>BBa_K1379007 short</partinfo> | <partinfo>BBa_K1379007 short</partinfo> | ||
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- Phelicase primers sequence was obtained from [https://www.sanger.ac.uk/ Wellcome Trust Sanger Institute], a British genomics and genetics research institute. | - Phelicase primers sequence was obtained from [https://www.sanger.ac.uk/ Wellcome Trust Sanger Institute], a British genomics and genetics research institute. | ||
| − | - both | + | - both sigmaX gene and Phelicase promoter were cloned from genomic DNA of Streptococcus pneumoniae NCTC7465 strain<br> |
- Constitutive promoter + RBS (BBa_K880005) was obtained from iGEM distribution kit 2014 | - Constitutive promoter + RBS (BBa_K880005) was obtained from iGEM distribution kit 2014 | ||
- Double terminator (BBa_B0015) was obtained from iGEM distribution kit 2013 | - Double terminator (BBa_B0015) was obtained from iGEM distribution kit 2013 | ||
Revision as of 08:46, 7 October 2014
σx Generator + PcomFA-E0240
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 359
Illegal BsaI.rc site found at 1550
Design Notes
None
Source
- Phelicase primers sequence was obtained from Wellcome Trust Sanger Institute, a British genomics and genetics research institute.
- both sigmaX gene and Phelicase promoter were cloned from genomic DNA of Streptococcus pneumoniae NCTC7465 strain
- Constitutive promoter + RBS (BBa_K880005) was obtained from iGEM distribution kit 2014
- Double terminator (BBa_B0015) was obtained from iGEM distribution kit 2013
- GFP generator (BBa_E0240) was obtained from iGEM distribution kit 2014