Difference between revisions of "Part:BBa K1341001"

Revision as of 05:08, 7 October 2014

PATH WAY TEST SYS IN BIO-COMPASS

This system has proved that we have successful insert the BBa_K1341000 in the Afl III enzyme site(which has designed in the No.5 node sequence before this path way linked).Besides, this system also have a BamH I site in the No.2(start) point, a Nco I site in the No.8(terminal） point. The TetR device (BBa_K1341003) and LacI device (BBa_K1341002) can insert in this measurement system according to the requirement. By using the aTc and IPTG, we can prove whether the path way is the right result exactly such as this(2-4-5-7-8) CHA TU PIAN 1

The analysis about the logic of the system

CHA TU PIAN 2 Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 11
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 180
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 837

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1341001 short</partinfo>
 This system has proved that we have successful insert the BBa_K1341000 in the Afl III enzyme site(which has designed in the No.5 node sequence before this path way linked).Besides, this system also have a BamH I site in the No.2(start) point, a Nco I site in the No.8(terminal） point. The TetR device (BBa_K1341003) and LacI device (BBa_K1341002) can insert in this measurement system according to the requirement. By using the aTc and IPTG, we can prove whether the path way is the right result exactly such as this(2-4-5-7-8)
 CHA TU PIAN 1