Difference between revisions of "Part:BBa K1362413:Design"

 
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===Source===
 
===Source===
  
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Part information and data come from Schmidt et al. [[#References|[1]]]. The sequence was acquired from Pietrokovski et al.  [[#References|[1]]] (Supplementary data: http://nar.oxfordjournals.org/content/suppl/2009/02/25/gkp095.DC1/nar-00060-s-2009-File008.pdf). Part DNA was obtained by DNA synthesis.
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Part information and data come from Schmidt et al. [[#References|[1]]]. The sequence was acquired from Pietrokovski et al.  [[#References|[2]]] (to be found in the supplementary data [[#References|[3]]]) Part DNA was obtained by DNA synthesis.
  
 
===References===
 
===References===
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[1] 1. Carvajal-Vallejos, P., Pallissé, R., Mootz, H. D. & Schmidt, S. R. Unprecedented rates and efficiencies revealed for new natural split inteins from metagenomic sources. J. Biol. Chem. 287, 28686–96 (2012).
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[2] Bareket Dassa, Nir London, Barry L. Stoddard, Ora Schueler-Furman, Shmuel Pietrokovski Fractured genes: a novel genomic arrangement involving new split inteins and a new homing endonuclease family Nucleic Acids Res. 2009 May; 37(8): 2560–2573. Published online 2009 March 5. doi: 10.1093/nar/gkp095
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[3] http://nar.oxfordjournals.org/content/suppl/2009/02/25/gkp095.DC1/nar-00060-s-2009-File008.pdf

Latest revision as of 21:00, 6 October 2014


Gp41-1 C-Intein cloning piece


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 28


Design Notes

This part represents only the intein sequence without including the standard splicing-site or polyglycine-linker overhangs.


Source

Part information and data come from Schmidt et al. [1]. The sequence was acquired from Pietrokovski et al. [2] (to be found in the supplementary data [3]) Part DNA was obtained by DNA synthesis.

References

[1] 1. Carvajal-Vallejos, P., Pallissé, R., Mootz, H. D. & Schmidt, S. R. Unprecedented rates and efficiencies revealed for new natural split inteins from metagenomic sources. J. Biol. Chem. 287, 28686–96 (2012).

[2] Bareket Dassa, Nir London, Barry L. Stoddard, Ora Schueler-Furman, Shmuel Pietrokovski Fractured genes: a novel genomic arrangement involving new split inteins and a new homing endonuclease family Nucleic Acids Res. 2009 May; 37(8): 2560–2573. Published online 2009 March 5. doi: 10.1093/nar/gkp095

[3] http://nar.oxfordjournals.org/content/suppl/2009/02/25/gkp095.DC1/nar-00060-s-2009-File008.pdf