Difference between revisions of "Part:BBa K1400003"
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<partinfo>BBa_K1400003 short</partinfo> | <partinfo>BBa_K1400003 short</partinfo> | ||
− | A single tet responsive promoter created by replacing the | + | A single tet responsive promoter created by removing the 2 most distal (from TATA box) gal4 sites, and replacing the last 2 gal4 sites in the gal promoter with tet sites. THe two mig1 sites were replaced as well. It was designed to have similar expression to our modified gal promoter (BBA_K1400002). |
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Revision as of 19:52, 6 October 2014
pTre(4) Single input tet responsive promoter
A single tet responsive promoter created by removing the 2 most distal (from TATA box) gal4 sites, and replacing the last 2 gal4 sites in the gal promoter with tet sites. THe two mig1 sites were replaced as well. It was designed to have similar expression to our modified gal promoter (BBA_K1400002).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 35
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 166