Difference between revisions of "Part:BBa K1362403:Design"
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===References=== | ===References=== | ||
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+ | [1] Lin Y, Li M, Song H, Xu L, Meng Q, et al. (2013) Protein Trans-Splicing of Multiple Atypical Split Inteins Engineered from Natural Inteins. PLoS ONE 8(4): e59516. doi:10.1371/journal.pone.0059516 http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0059516 |
Latest revision as of 17:33, 6 October 2014
SspDnaX C-Intein cloning piece
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part represents only the intein sequence without including the standard splicing-site or polyglycine-linker overhangs.
Source
Part was made using by DNA synthesis. The Sequence is obtained from Liu et al.[1]
References
[1] Lin Y, Li M, Song H, Xu L, Meng Q, et al. (2013) Protein Trans-Splicing of Multiple Atypical Split Inteins Engineered from Natural Inteins. PLoS ONE 8(4): e59516. doi:10.1371/journal.pone.0059516 http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0059516