Difference between revisions of "Part:BBa K1362403:Design"
(→References) |
|||
(2 intermediate revisions by the same user not shown) | |||
Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
− | This part represents only the intein sequence without | + | This part represents only the intein sequence without including the standard splicing-site or polyglycine-linker overhangs. |
− | + | ||
− | + | ||
===Source=== | ===Source=== | ||
Line 16: | Line 14: | ||
===References=== | ===References=== | ||
+ | |||
+ | [1] Lin Y, Li M, Song H, Xu L, Meng Q, et al. (2013) Protein Trans-Splicing of Multiple Atypical Split Inteins Engineered from Natural Inteins. PLoS ONE 8(4): e59516. doi:10.1371/journal.pone.0059516 http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0059516 |
Latest revision as of 17:33, 6 October 2014
SspDnaX C-Intein cloning piece
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part represents only the intein sequence without including the standard splicing-site or polyglycine-linker overhangs.
Source
Part was made using by DNA synthesis. The Sequence is obtained from Liu et al.[1]
References
[1] Lin Y, Li M, Song H, Xu L, Meng Q, et al. (2013) Protein Trans-Splicing of Multiple Atypical Split Inteins Engineered from Natural Inteins. PLoS ONE 8(4): e59516. doi:10.1371/journal.pone.0059516 http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0059516