Difference between revisions of "Part:BBa K1403000:Design"

(→‎Design Notes)
(→‎Design Notes)
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The designed was made according to interlab study requirements. The promoter and the vector have been taken from [[Part:BBa_K823005|BBa_K823005]], cut with XbeI and SpeI. The GFP coding sequences as well as RBS and terminators have been cut out from [[Part:BBa_E0240|BBa_E0240]] also with XbeI and SpeI and introduced to the cut promoter + backbone.
 
The designed was made according to interlab study requirements. The promoter and the vector have been taken from [[Part:BBa_K823005|BBa_K823005]], cut with XbeI and SpeI. The GFP coding sequences as well as RBS and terminators have been cut out from [[Part:BBa_E0240|BBa_E0240]] also with XbeI and SpeI and introduced to the cut promoter + backbone.
 
We used heatshock competent NEB cels.
 
We used heatshock competent NEB cels.
−
For more details please check: [
+
For more details please check: [http://2014.igem.org/Team:Paris_Bettencourt/Project/Interlab_Study# Interlab study]
−
http://2014.igem.org/Team:Paris_Bettencourt/Project/Interlab_Study# Interlab study]
+
  
 
===Source===
 
===Source===

Revision as of 16:25, 6 October 2014


GFP generator in pSB1C3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 706


Design Notes

The designed was made according to interlab study requirements. The promoter and the vector have been taken from BBa_K823005, cut with XbeI and SpeI. The GFP coding sequences as well as RBS and terminators have been cut out from BBa_E0240 also with XbeI and SpeI and introduced to the cut promoter + backbone. We used heatshock competent NEB cels. For more details please check: [http://2014.igem.org/Team:Paris_Bettencourt/Project/Interlab_Study# Interlab study]

Source

kit 2014 Plate 1, Well 20K Plate 2, Well 24B

References