Difference between revisions of "Part:BBa K1403002"

 
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<partinfo>BBa_K1403002 short</partinfo>
 
<partinfo>BBa_K1403002 short</partinfo>
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agaA is an enzyme present in Corynebacterium striatum that is the main responsible for body odor. It metabolizes 3-methyl-2-hexenoic acid into 3-hydroxy-3-methylhexanoic acid, that is a sulphure volatile compound.
 
agaA is an enzyme present in Corynebacterium striatum that is the main responsible for body odor. It metabolizes 3-methyl-2-hexenoic acid into 3-hydroxy-3-methylhexanoic acid, that is a sulphure volatile compound.
  
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Name: BBa_K1403002
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This part contains an agaA sequence codon optimized for E coli 12 (IDT online tool). We checked with Geneious that there were no restriction sites for EcoRI, SpeI, XbaI and PstI.
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Description: Biobrick submission of agaA expression construct
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Resistances : Cm
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Backbone : pSB1C3
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Parts: Pcons - RBS - agaA - his tag
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Design date:  11/June/2014
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This part contains:
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1) Plasmid pSB1C3: High copy BioBrick assembly plasmid. Constitutive expression. To use in E coli
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2) agaA construct:
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- Promoter BBa_J23108 from the Anderson's promoter collection
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- RBS from the RBS Calculator of Salis lab specific for E coli
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- BioBrick Prefix + Scar from the iGEM Parts webpage
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- agaA sequence codon optimized for E coli 12 (IDT online tool). We checked with Geneious that there were no restriction sites for EcoRI, SpeI, XbaI and PstI.
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- Histidine tag: 6 Histidines in C-terminal were added
 
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-Stop codon
 
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-BioBrick scar + BioBrick suffix from the iGEM Parts webpage
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 11:47, 6 October 2014

AgaA expression (3M2H-> 3H3MH)

agaA is an enzyme present in Corynebacterium striatum that is the main responsible for body odor. It metabolizes 3-methyl-2-hexenoic acid into 3-hydroxy-3-methylhexanoic acid, that is a sulphure volatile compound.

This part contains an agaA sequence codon optimized for E coli 12 (IDT online tool). We checked with Geneious that there were no restriction sites for EcoRI, SpeI, XbaI and PstI.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 288
    Illegal BamHI site found at 519
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 181
  • 1000
    COMPATIBLE WITH RFC[1000]