Difference between revisions of "Part:BBa K1520000:Design"

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===Design Notes===
 
===Design Notes===
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This is a sequence of a protein involved in degradation of microsystin,short for mrlD.In detail,it is a kind of membrane protein,and it is able to make bacteria absorb microsystin in to itself.With another enzyme,mrlA,microsystin will be successfully cleaned up.
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mrlD is a gene involved in degradation of microsystin. The MlrD protein belongs to a protein family which is predicted to carry small peptides across the bacterial cell wall and it is therefore possible that this protein facilitates the transport of microcystin. The taking in process would facility another protein,MrlA,which is an enzyme of microcystin to clean up microcystin successfully.
  
 
===Source===
 
===Source===
  
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Mlrd
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It is synthetic.The gene was discovered in Sphingopyxis sp. C-1,and the sequence of it can be obtained from genebank >gi|349500571|dbj|AB591070.1|.
  
 
===References===
 
===References===
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Bourne D G, Riddles P, Jones G J, et al. Characterisation of a gene cluster involved in bacterial degradation of the cyanobacterial toxin microcystin LR[J]. Environmental toxicology, 2001, 16(6): 523-534.

Latest revision as of 17:52, 5 October 2014


MlrD.The protein is involved in microcystin degradation.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1042


Design Notes

mrlD is a gene involved in degradation of microsystin. The MlrD protein belongs to a protein family which is predicted to carry small peptides across the bacterial cell wall and it is therefore possible that this protein facilitates the transport of microcystin. The taking in process would facility another protein,MrlA,which is an enzyme of microcystin to clean up microcystin successfully.

Source

It is synthetic.The gene was discovered in Sphingopyxis sp. C-1,and the sequence of it can be obtained from genebank >gi|349500571|dbj|AB591070.1|.

References

Bourne D G, Riddles P, Jones G J, et al. Characterisation of a gene cluster involved in bacterial degradation of the cyanobacterial toxin microcystin LR[J]. Environmental toxicology, 2001, 16(6): 523-534.