Difference between revisions of "Part:BBa K1537024"

Revision as of 14:59, 5 October 2014

HPS-PHI

The ribulose monophosphate (RuMP) pathway is one of the HCHO-fixation pathways found in microorganisms called methylotrophs, which utilize one-carbon compounds as the sole carbon source. The key enzymes of this pathway are 3-hexulose-6-phosphate synthase (HPS), which fixes HCHO to D-ribulose-5-phosphate (Ru5P) to produce D-arabino-3-hexulose 6-phosphate (Hu6P), and 6-phospho-3- hexuloisomerase (PHI), which converts Hu6P to fructose 6-phosphate (F6P).The two key enzymes work in chloroplast both.We will use fusion expression to conduct heterologous expression in tobacco.

Fig.1 Schematic Representation of the Bacterial RuMP Pathway and the Plant Calvin-Benson Cycle. HPS and PHI denote 3-hexulose-6-phosphate synthase and 6-phospho-3-hexuloisomerase respectively. The abbreviations for several sugar phosphates are as follows: Ru5P, ribulose 5-phosphate; Hu6P, hexulose 6-phosphate; F6P, fructose 6-phosphate; FBP, fructose 1,6-bisphosphate;RuBP,ribulose1,5-bisphosphate;3-PGA,3-phosphoglyce-rate. The other metabolites in the pathway are symbolized merely by their carbon numbers for simplicity.

Fig.2 AB1 and AB2, two lines transformed with both rmpA(the gene for HPS) and rmpB(the gene for PHI); CK, a negativecontrol line transformed with the empty vector.

References Li-mei CHEN, Hiroya YURIMOTO,3 Kun-zhi LI, Izumi ORITA,Motomu AKITA,Nobuo KATO,Yasuyoshi SAKAI, and Katsura IZUI(2010).Assimilation of Formaldehyde in Transgenic Plants Due to the Introduction of the Bacterial Ribulose Monophosphate Pathway Genes. Biosci. Biotechnol. Biochem.74 (3), 627–635

Sequence and Features