Difference between revisions of "Part:BBa K1351026:Design"

 
 
(One intermediate revision by the same user not shown)
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1351026 short</partinfo>
 
<partinfo>BBa_K1351026 short</partinfo>
  
 
<partinfo>BBa_K1351026 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1351026 SequenceAndFeatures</partinfo>
−
 
−
 
−
===Design Notes===
 
−
none
 
−
 
  
  
Line 20: Line 14:
  
 
===References===
 
===References===
 +
Tjalsma, H., A. Bolhuis, J. D. Jongbloed, S. Bron and J. M. van Dijl. "Signal Peptide-Dependent Protein Transport in Bacillus Subtilis: A Genome-Based Survey of the Secretome." Microbiol Mol Biol Rev 64, no. 3 (2000): 515-47. [http://www.ncbi.nlm.nih.gov/pubmed/10974125 PubMed]
 +
 +
Yamamoto, H., S. Kurosawa and J. Sekiguchi. "Localization of the Vegetative Cell Wall Hydrolases Lytc, Lyte, and Lytf on the Bacillus Subtilis Cell Surface and Stability of These Enzymes to Cell Wall-Bound or Extracellular Proteases." J Bacteriol 185, no. 22 (2003): 6666-77. [http://www.ncbi.nlm.nih.gov/pubmed/14594841 PubMed]

Latest revision as of 12:39, 5 October 2014

Signal peptide of B. subtilis major autolysin LytC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Source

This part was generated by amplification from BioBrick BBa_K1351006 with the primers listed below, followed by digestion with EcoRI and PstI and ligation into pSB1C3.

LytC_ENX_SD_Ngo_fwd: gatcGAATTCgcggccgctTCTAGAgtaaggaggaaGCCGGC TTGCGTTCTTATATAAAAGTCCTAACAATGTG

LytcSP_SNP_Age_rev: gatcCTGCAGcggccgctACTAGTattaACCGGT ATCGGCCAAAGCTGTTGGC

References

Tjalsma, H., A. Bolhuis, J. D. Jongbloed, S. Bron and J. M. van Dijl. "Signal Peptide-Dependent Protein Transport in Bacillus Subtilis: A Genome-Based Survey of the Secretome." Microbiol Mol Biol Rev 64, no. 3 (2000): 515-47. [http://www.ncbi.nlm.nih.gov/pubmed/10974125 PubMed]

Yamamoto, H., S. Kurosawa and J. Sekiguchi. "Localization of the Vegetative Cell Wall Hydrolases Lytc, Lyte, and Lytf on the Bacillus Subtilis Cell Surface and Stability of These Enzymes to Cell Wall-Bound or Extracellular Proteases." J Bacteriol 185, no. 22 (2003): 6666-77. [http://www.ncbi.nlm.nih.gov/pubmed/14594841 PubMed]