Difference between revisions of "Part:BBa K759001:Experience"
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Escherichia coli bacterial culture containing plasmids BBa_K759001 (coding region of Ag43 only), was inoculated overnight in LB-chloramphenicol medium in a shaking water bath at 370C. Plasmid mini preps were conducted with a Qiagen plasmid mini prep kit. Single and double restriction digests were carried out accordingly: | Escherichia coli bacterial culture containing plasmids BBa_K759001 (coding region of Ag43 only), was inoculated overnight in LB-chloramphenicol medium in a shaking water bath at 370C. Plasmid mini preps were conducted with a Qiagen plasmid mini prep kit. Single and double restriction digests were carried out accordingly: | ||
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| + | [[File:Restriction_digest_table.png|200px|thumb|left|alt text]] | ||
Revision as of 22:28, 3 October 2014
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K759001
User Reviews
UNIQ8fd286804cd40052-partinfo-00000000-QINU
UNIQ8fd286804cd40052-partinfo-00000001-QINU
• Aim – to verify Assembly Standard 10 compliance
• Methods:
Standard restriction digest and gel electrophoresis
Escherichia coli bacterial culture containing plasmids BBa_K759001 (coding region of Ag43 only), was inoculated overnight in LB-chloramphenicol medium in a shaking water bath at 370C. Plasmid mini preps were conducted with a Qiagen plasmid mini prep kit. Single and double restriction digests were carried out accordingly:
