Difference between revisions of "Part:BBa K1351000"
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Displayed on the surface of ''Bacillus subtilis'', it should enable ''B. subtilis'' to bind specifically to ''S. aureus''.
 
Displayed on the surface of ''Bacillus subtilis'', it should enable ''B. subtilis'' to bind specifically to ''S. aureus''.
  
−
This part was generated in RFC25 standard with the following prefix and suffix: <br>
+
This part was generated in amodified version of RFC25, where a strong Shine Dalgarno Sequence (SD) is included, and has the following prefix and suffix: <br>
−
ENX_SD_Ngo_fwd: 5' '''GAATTC'''-'''GCGGCCGC'''-T-'''TCTAGA'''-GT-<u>AAGGAGG</u>-A-'''GCCGGC'''-ATG 3'<br>
+
{|
−
PNS_Age_rev: 5' '''CTGCAG'''-'''CGGCCG <u>C'''-T-'''A</u> <u>CTA</u>GT'''-A<u>TTA</u>-'''ACCGGT''' 3' <br>
+
|prefix with EcoRI, NotI, XbaI, SD and NgoMIV:  
−
Restriction sites in bold, Shine-Dalgarno sequence and stop codons underlined.
+
|<span style="color:blue">GAATTC</span><span style="color:green">GCGGCCGC</span>T<span style="color:red">TCTAGA</span>GT<u>AAGGAGG</u>A<span style="color:orange">GCCGGC</span>  
−
 
+
|-
 +
|suffix with AgeI, SpeI, NotI and PstI:
 +
|<span style="color:orange">ACCGGT</span><u>TAA</u>T<span style="color:red">AC<u>TAG</u><u>T</u></span><u>A</u><span style="color:green"><u>G</u>CGGCCGC</span><span style="color:blue">CTGCAG</span>
 +
|}
 +
Sites of restriction enzymes generating compatible overhangs have the same color:<br>
 +
<span style="color:blue">EcoRI</span> and <span style="color:blue">PstI</span> in blue, <span style="color:green">NotI</span> in green, <span style="color:red">XbaI</span> and <span style="color:red">PstI</span> in red, <span style="color:orange">NgoMIV</span> and <span style="color:orange">AgeI</span> in orange. Shine-Dalgarno sequence and stop codons are underlined.
  
 +
<strong>blue</strong>
  
  

Revision as of 16:35, 3 October 2014

beta-Neurexin-derived peptide binding to S. aureus adhesin SdrC

Short peptide of 11 amino acids corresponding to the N-terminus of the human protein neurexin beta-1. It includes the binding site (HHIHHFH) for the Staphylococcus aureus adhesin SdrC. Displayed on the surface of Bacillus subtilis, it should enable B. subtilis to bind specifically to S. aureus.

This part was generated in amodified version of RFC25, where a strong Shine Dalgarno Sequence (SD) is included, and has the following prefix and suffix:

prefix with EcoRI, NotI, XbaI, SD and NgoMIV: GAATTCGCGGCCGCTTCTAGAGTAAGGAGGAGCCGGC
suffix with AgeI, SpeI, NotI and PstI: ACCGGTTAATACTAGTAGCGGCCGCCTGCAG

Sites of restriction enzymes generating compatible overhangs have the same color:
EcoRI and PstI in blue, NotI in green, XbaI and PstI in red, NgoMIV and AgeI in orange. Shine-Dalgarno sequence and stop codons are underlined.

blue


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]