Difference between revisions of "Part:BBa K1529310"
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To characterize the function of this <i>rhl(LR)</i> promoter, we constructed a part Prhl(LR)_GFP (<partinfo>BBa_K1529311</partinfo>) by inserting the <i>rhl(LR)</i> promoter, upstream of a GFP coding sequence.
 
To characterize the function of this <i>rhl(LR)</i> promoter, we constructed a part Prhl(LR)_GFP (<partinfo>BBa_K1529311</partinfo>) by inserting the <i>rhl(LR)</i> promoter, upstream of a GFP coding sequence.
  
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[[Image:titech2013_parts_K1139150_main_Fig1.jpg|thumb|center|250px|<b>Fig. 1.</b> Our newly designed  promoter]]
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[[Image:titech2014_parts_rhlpromoter_main_Fig1.jpg|thumb|center|250px|<b>Fig. 1.</b> Our newly designed  promoter]]
  
 
By using the reporter cells that contain Prhl(LR)_GFP, we measured the fluorescence intensity of the cells induced by C4HSL (Fig. 2).  
 
By using the reporter cells that contain Prhl(LR)_GFP, we measured the fluorescence intensity of the cells induced by C4HSL (Fig. 2).  
 
We saw that our new <i>rhl(LR)</i> promoter was actually activated by C4HSL through an induction assay (Fig. 3).
 
We saw that our new <i>rhl(LR)</i> promoter was actually activated by C4HSL through an induction assay (Fig. 3).
  
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[[Image:titech2013_parts_K1139150_main_Fig2.jpg|thumb|left|310px|<b>Fig. 2.</b> Fluorescence intensity detected by flow cytometer]]
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[[Image:titech2014_parts_GFPassay_main_Fig2.jpg|thumb|center|310px|<b>Fig. 2.</b> Fluorescence intensity detected by flow cytometer]]
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[[Image:titech2013_parts_K1139150_main_Fig3.jpg|thumb|none|240px|<b>Fig. 3.</b> Comparison of N99 and JM2.300]]
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Revision as of 11:51, 3 October 2014

Prhl(LR)

We newly developed the rhl(LR) promoter (BBa_K1529310) which is activated by C4HSL (Fig. 1).
To characterize the function of this rhl(LR) promoter, we constructed a part Prhl(LR)_GFP (BBa_K1529311) by inserting the rhl(LR) promoter, upstream of a GFP coding sequence.

File:Titech2014 parts rhlpromoter main Fig1.jpg
Fig. 1. Our newly designed promoter

By using the reporter cells that contain Prhl(LR)_GFP, we measured the fluorescence intensity of the cells induced by C4HSL (Fig. 2). We saw that our new rhl(LR) promoter was actually activated by C4HSL through an induction assay (Fig. 3).

File:Titech2014 parts GFPassay main Fig2.jpg
Fig. 2. Fluorescence intensity detected by flow cytometer



For more information, see [http://2013.igem.org/Team:Tokyo_Tech/Experiment/RM-lac_Hybrid_Promoter_Assay our work in Tokyo_Tech 2013 wiki].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]