Difference between revisions of "Part:BBa K1439002"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1439002 short</partinfo>
 
<partinfo>BBa_K1439002 short</partinfo>
  
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Once the conjugation take place,the recipient bacterium will obtain the reporter gene which gives us a signal to distinguish whether the conjugation has happened as well as the conjugation efficiency easily through observation. In addition, it facilitates the Follow-up experiments on the conjugation rate, conjugation time, and conjugation efficiency between different strains.
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[OriTRP4]+[RFP]
  
  

Revision as of 08:30, 2 October 2014

This part contains a reporter gene BBa_J04450, combined with OriTR. Used to test plasmid mobility.

[OriTRP4]+[RFP]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 117
    Illegal NgoMIV site found at 127
    Illegal AgeI site found at 1160
    Illegal AgeI site found at 1272
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 19