Difference between revisions of "Part:BBa K1429002"
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IP free cyan fluorescent protein.  We have a ribosome binding site (RBS).
 
IP free cyan fluorescent protein.  We have a ribosome binding site (RBS).
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 +
Background
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 +
Making sure the inserts are here from the colony PCR results - are the GFP and RFP inserts present?
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 +
 +
 +
Digest each plasmid with EcoRI/PstI. Incubate 15' at 37C.
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 +
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Run entire digest on 0.8% gel.
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 +
 +
Results
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 +
These were slightly confounding. What I *think* happened is that the buffer wasn't optimal for one of the enzymes, so the digest wasn't run to completion. Why do I think this?
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 01:13, 1 October 2014

Cyan Fluorescent Protein (CFP) "Cindy Lou" coding region, intellectual property-free

IP free cyan fluorescent protein. We have a ribosome binding site (RBS).

Background

Making sure the inserts are here from the colony PCR results - are the GFP and RFP inserts present?


Digest each plasmid with EcoRI/PstI. Incubate 15' at 37C.


Run entire digest on 0.8% gel.


Results

These were slightly confounding. What I *think* happened is that the buffer wasn't optimal for one of the enzymes, so the digest wasn't run to completion. Why do I think this?

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 74
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2