Difference between revisions of "Part:BBa F2620"
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'''Description'''<br> | '''Description'''<br> | ||
| − | A transcription factor [LuxR] that is active in the presence of cell-cell signaling molecule [[3OC6HSL| | + | A transcription factor [LuxR] (''<partinfo>C0062</partinfo>'') that is active in the presence of cell-cell signaling molecule [[3OC6HSL|3OC<sub>6</sub>HSL]] is controlled by a tetR regulatable operator (''<partinfo>BBa_R0040</partinfo>''. ''Device input'' is [[3OC6HSL|3OC<sub>6</sub>HSL]]. ''Device output'' is [[PoPS]] from a LuxR-regulated operator. |
'''Usage'''<br> | '''Usage'''<br> | ||
Revision as of 19:46, 18 September 2006
A work in progress...
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Authors: Barry Canton [bcanton@mit.edu], Anna Labno [labnoa@mit.edu] Last Update: Sept. 15th, 2006
Description
A transcription factor [LuxR] (BBa_C0062) that is active in the presence of cell-cell signaling molecule 3OC6HSL is controlled by a tetR regulatable operator (BBa_R0040. Device input is 3OC6HSL. Device output is PoPS from a LuxR-regulated operator.
Usage
Full PoPS output at high 3OC6HSL levels and high plasmid copy [e.g., pSB1A2] results in a reduced cell growth rate (see Load section). If used in a cell containing TetR then a second input signal such as [http://openwetware.org/wiki/ATc aTc] can be used to produce a logical AND function.
Characteristics
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Key Subparts
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| Transfer Function |
Specificity |
| Latency |
Stability |
Demand
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Stability
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Compatibility
Device has been shown to work in BBa_V1002, MG1655 and BBa_V1001
Device has been shown to work on pSB3K3 and pSB3K3
Device has been shown to work with BBa_E0430,BBa_E0434,BBa_E0240
Crosstalk with devices using 3OC6HSL or add in the other molecule names here.
Crosstalk with systems containing TetR (BBa_C0040)
Characterization Conditions
All characterization was performed on pSB3K3 using MG1655 as the characterization chassis. Cultures were grown in [http://openwetware.org/wiki/Endy:M9_media/supplemented supplemented M9 media] at 37C. Full characterization protocols can be found here.
