Difference between revisions of "Part:BBa K1465201:Design"

(→‎Design Notes)
(→‎Design Notes)
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
Gibson-Assembly
 
−
 
 
<html>
 
<html>
−
Primer <a href="http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#pSB1C3_pre_prkA" target="_blank">pSB1C3_pre_prkA</a> and <a href="http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#pSB1C3_suf_prkA" target="_blank">pSB1C3_suf_prkA</a>
+
<a href="http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#Gibson" target="_blank">Gibson-Assembly</a> using the Primer <a href="http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#pSB1C3_pre_prkA" target="_blank">pSB1C3_pre_prkA</a> and <a href="http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#pSB1C3_suf_prkA" target="_blank">pSB1C3_suf_prkA</a>
 
</html>
 
</html>
  

Revision as of 21:17, 29 September 2014


Phosphoribulokinase prkA from Synechococcus elongatus


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1009
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 198
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 657
    Illegal AgeI site found at 928
    Illegal AgeI site found at 999
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Gibson-Assembly using the Primer pSB1C3_pre_prkA and pSB1C3_suf_prkA

Source

Gensynthesis (gBlocks)

References