Difference between revisions of "Part:BBa K1555000"

 
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<partinfo>BBa_K1555000 short</partinfo>
 
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This part is our testing part.The copa is one of the candidates of our copper sensor.For comparing with other two sequences,marRAB and cueo,we linked a reporter(BBa_E0840) follow the promoter of copa.With the concentration of copper rises, the expression of downstream gfp coding gene increases.We designed two experiments to test those candidates' sensitivity and specificity individually by measure fluorescence intensity.
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This part is our testing part.The copa is one of the candidates of our copper sensor.For comparing with other two sequences,marRAB and cueo,we linked a reporter(BBa_E0840) follow the promoter of copa.With the concentration of copper rises, the expression of downstream gfp coding gene increases.We designed two experiments to test those candidates' sensitivity and specificity individually by measuring fluorescence intensity.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 19:19, 28 September 2014

The copa promoter wth a gfp reporter

This part is our testing part.The copa is one of the candidates of our copper sensor.For comparing with other two sequences,marRAB and cueo,we linked a reporter(BBa_E0840) follow the promoter of copa.With the concentration of copper rises, the expression of downstream gfp coding gene increases.We designed two experiments to test those candidates' sensitivity and specificity individually by measuring fluorescence intensity.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 914