Difference between revisions of "Part:BBa K1555000:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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For guaranteeing our sensor is simple but copper-sensitive,we need to find a operator which directly or indirectly regulated by copper in copa sequence.
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For guaranteeing our sensor is simple but copper-sensitive,we select upstream 17bp-249bp of ''copa'' sequence as our candidates.It includes a sequence which indirectly regulated by copper through copper-responsive protein CueR.
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===Source===
 
===Source===

Revision as of 19:12, 28 September 2014


The copa promoter wth a gfp reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 914


Design Notes

For guaranteeing our sensor is simple but copper-sensitive,we select upstream 17bp-249bp of copa sequence as our candidates.It includes a sequence which indirectly regulated by copper through copper-responsive protein CueR.

Source

We attained this sensor from E.coli's genome.

References