Difference between revisions of "Part:BBa S03595:Experience"
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Once the Double Forward Terminator [[https://parts.igem.org/wiki/index.php/Part:BBa_B0015 BBa_B0015]] is placed upstream of RBS-TetR, the cells are no longer tetracycline resistant. We conclude that the Double Forward Terminator is sufficient to block read-through transcription, allowing control over the expression of the downstream coding region. | Once the Double Forward Terminator [[https://parts.igem.org/wiki/index.php/Part:BBa_B0015 BBa_B0015]] is placed upstream of RBS-TetR, the cells are no longer tetracycline resistant. We conclude that the Double Forward Terminator is sufficient to block read-through transcription, allowing control over the expression of the downstream coding region. | ||
| − | Construction of an "insulator vector" where the double terminator is placed before the BioBrick prefix and after the BioBrick suffix (in the reverse orientation) is currently | + | Construction of an "insulator vector" where the double terminator is placed before the BioBrick prefix and after the BioBrick suffix (in the reverse orientation) is currently under way. |
===User Reviews=== | ===User Reviews=== | ||
Revision as of 22:26, 14 September 2006
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_S03595
This part was constructed to test the capacity of the Double Forward Terminator [BBa_B0015] to block read-through transcription.
Part [BBa_S03562], which contains TetR with a ribosomal binding site, is sufficient to convey tetracycline resistance in the absence of a promoter. We suspected that TetR might be expressed via read-through transcription from the carrier vector.
Consistent with this observation, the following parts are also expressed in the absence of an upstream promoter:
- Insert list here
Once the Double Forward Terminator [BBa_B0015] is placed upstream of RBS-TetR, the cells are no longer tetracycline resistant. We conclude that the Double Forward Terminator is sufficient to block read-through transcription, allowing control over the expression of the downstream coding region.
Construction of an "insulator vector" where the double terminator is placed before the BioBrick prefix and after the BioBrick suffix (in the reverse orientation) is currently under way.
User Reviews
UNIQ671d5eaf91ee1038-partinfo-00000000-QINU UNIQ671d5eaf91ee1038-partinfo-00000001-QINU