Difference between revisions of "Part:BBa K1554002"

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(→‎Usage and Biology)
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__NOTOC__
 
__NOTOC__
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<partinfo>BBa_K1554001 short</partinfo>
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<partinfo>BBa_K1554002 short</partinfo>
  
 
HarFAR-3 fatty acid reductase from ''Helicoverpa armigera'' with an ER retention signal (KKYR) attached to the C-terminal end. Codon optimization was perfomed for ''Nicotiana benthamiana''.
 
HarFAR-3 fatty acid reductase from ''Helicoverpa armigera'' with an ER retention signal (KKYR) attached to the C-terminal end. Codon optimization was perfomed for ''Nicotiana benthamiana''.
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The HarFAR protein is a fatty acid reductase coming from ''Helicoverpa armigera'' which transforms fatty acids into fatty alcohols.
 
The HarFAR protein is a fatty acid reductase coming from ''Helicoverpa armigera'' which transforms fatty acids into fatty alcohols.
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It can be used with part [https://parts.igem.org/Part:BBa_K1554002 Part:BBa_K1554002]
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Our team used it in with [https://parts.igem.org/Part:BBa_K1554002 Part:BBa_K1554002] to produce the insect pheromone Z11-16:OH in ''Nicotiana benthamiana'' using palmitate:CoA as precursor. It was also used with [https://parts.igem.org/Part:BBa_K1554003 Part:BBa_K1554003] to transform Z11-16:OH into antoher insect pheromone, Z11-16:OAc.
  
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1554001 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K1554002 SequenceAndFeatures</partinfo>
  
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K1554001 parameters</partinfo>
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<partinfo>BBa_K1554002 parameters</partinfo>
 
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Revision as of 12:13, 28 September 2014

HarFAR

HarFAR-3 fatty acid reductase from Helicoverpa armigera with an ER retention signal (KKYR) attached to the C-terminal end. Codon optimization was perfomed for Nicotiana benthamiana.

Usage and Biology

The HarFAR protein is a fatty acid reductase coming from Helicoverpa armigera which transforms fatty acids into fatty alcohols. Our team used it in with Part:BBa_K1554002 to produce the insect pheromone Z11-16:OH in Nicotiana benthamiana using palmitate:CoA as precursor. It was also used with Part:BBa_K1554003 to transform Z11-16:OH into antoher insect pheromone, Z11-16:OAc.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1104
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 929