Difference between revisions of "Part:BBa K1331004"
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<partinfo>BBa_K1331004 short</partinfo> | <partinfo>BBa_K1331004 short</partinfo> | ||
− | RhlB together with RhlA encodes Rhamnosyltransferase I for mono-rahmnolipids biosynthesis in Pseudomonas aeruginosa. This part includes | + | RhlB together with RhlA encodes Rhamnosyltransferase I for mono-rahmnolipids biosynthesis in ''Pseudomonas aeruginosa''. This part includes a part of upstream noncoding gene and a part of downstreanm noncoding gene of RhlB. Two mutations have been done to remove the PstI restriction sites contained in the coding sequence, which won't change the amino acid sequence. |
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Revision as of 08:08, 26 September 2014
Modified rhlB coding sequence from Pseudomonas aeruginosa SQ6
RhlB together with RhlA encodes Rhamnosyltransferase I for mono-rahmnolipids biosynthesis in Pseudomonas aeruginosa. This part includes a part of upstream noncoding gene and a part of downstreanm noncoding gene of RhlB. Two mutations have been done to remove the PstI restriction sites contained in the coding sequence, which won't change the amino acid sequence.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1144
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 37
Illegal NgoMIV site found at 758
Illegal NgoMIV site found at 871 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 387