Difference between revisions of "Part:BBa K1513000:Design"
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<partinfo>BBa_K1513000 short</partinfo> | <partinfo>BBa_K1513000 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
− | We hoped to adopt a quorum sensing system to accelerate expression of the reporter genes downstream the biosensor. However,the former quorum sensing part(BBa_J09855) required outer AHL. To | + | We hoped to adopt a quorum sensing system to accelerate expression of the reporter genes downstream the biosensor. However,the former quorum sensing part(BBa_J09855) required outer AHL. To fix this , we improved it by adding a luxI gene. |
===Source=== | ===Source=== |
Latest revision as of 06:18, 26 September 2014
A quorum sensing system which can produce auto-inducer (AHL) itself.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1710
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 987
Design Notes
We hoped to adopt a quorum sensing system to accelerate expression of the reporter genes downstream the biosensor. However,the former quorum sensing part(BBa_J09855) required outer AHL. To fix this , we improved it by adding a luxI gene.
Source
partJ09855、partK081008