Difference between revisions of "Part:BBa K1399010"

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== Plac-RFP(AAV) ==
 
== Plac-RFP(AAV) ==
  
 
Lactose/IPTG inducible promoter with RFP reporter tagged with AAV-ssrA degradation tag followed by terminator.
 
Lactose/IPTG inducible promoter with RFP reporter tagged with AAV-ssrA degradation tag followed by terminator.
 
The tagged RFP is actively degraded within cell, thus it provides better temporal resolution of red fluorescence and promoter activity.
 
The tagged RFP is actively degraded within cell, thus it provides better temporal resolution of red fluorescence and promoter activity.
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1399010 SequenceAndFeatures</partinfo>

Revision as of 12:21, 25 September 2014

Plac-RFP(AAV)

Lactose/IPTG inducible promoter with RFP reporter tagged with AAV-ssrA degradation tag followed by terminator. The tagged RFP is actively degraded within cell, thus it provides better temporal resolution of red fluorescence and promoter activity.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 781
    Illegal AgeI site found at 893
  • 1000
    COMPATIBLE WITH RFC[1000]