Difference between revisions of "Part:BBa K1333204:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | This is a composite part including | + | This is a composite part including plac Or2-62 and GFP generator. Use a strong RBS and GFP to tell the interactional degree between baits and targets in Bacterial Two-hybrid System. |
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===Source=== | ===Source=== | ||
Revision as of 13:15, 24 September 2014
Or2-RBS-GFP-terminator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 741
Design Notes
This is a composite part including plac Or2-62 and GFP generator. Use a strong RBS and GFP to tell the interactional degree between baits and targets in Bacterial Two-hybrid System.
Source
Mutated LGF2 at site 78 from Asp to Ala and fused it onto lambda cI.