Difference between revisions of "Part:BBa K1467100"
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Revision as of 10:12, 24 September 2014


RFP coding device - Golden Gate Module Flipper

The colonies are clearly red in color under natural light after about 18 hours. Smaller colonies are visibly red under UV. The RFP part does not contain a degradation tag and the RBS is strong. LacI sensitive CAP sensitive This part is a variation of Part:BBa_J04450 with additional flanking sequences recognised by the enzyme BsaI that allow standard GoldenGate Modular Cloning (MoClo) Promoter+5' untranslated leader (Pro+5U) modules to be 'flipped' in a one-pot, one-step, digestion-ligation reaction with BsaI, thus converting the MoClo part to a BioBrick part. When cloning is successful the colonies become white as the RFP sequence is replaced by the new part.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 793
    Illegal AgeI site found at 905
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1082
    Illegal BsaI.rc site found at 6