Difference between revisions of "Part:BBa K1333201:Design"

 
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===Design Notes===
 
===Design Notes===
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Mutation of GAC to GCC LGF2 at site 78.
+
The LGF2 is mutated from GAC to GCC at site 78.
−
 
+
−
 
+
  
 
===Source===
 
===Source===
  
−
From Agilent Technologies' BacterioMatch ⅡTwo-Hybrid System Vector Kit.
+
It is from Agilent Technologies' BacterioMatch ⅡTwo-Hybrid System Vector Kit and modified by Site-Directed Mutagenesis(SDM).
  
 
===References===
 
===References===

Latest revision as of 04:19, 24 September 2014


lambda cI-LGF2(D78A)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 712
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 767
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The LGF2 is mutated from GAC to GCC at site 78.

Source

It is from Agilent Technologies' BacterioMatch ⅡTwo-Hybrid System Vector Kit and modified by Site-Directed Mutagenesis(SDM).

References