Difference between revisions of "Part:BBa K1365000:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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Used primers:
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forward - 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAGTACAAAAGATTTTAA-3'
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reverse - 5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATTTGCTTACGTGAA-3'
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to PCR NisA from the genome and add the BioBrick prefix and suffix.
  
 
===Source===
 
===Source===

Revision as of 08:39, 23 September 2014


NisA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Used primers: forward - 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAGTACAAAAGATTTTAA-3' reverse - 5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATTTGCTTACGTGAA-3' to PCR NisA from the genome and add the BioBrick prefix and suffix.

Source

Obtained by PCR on the genome of L. lactis NZ9700, a nisin producing strain.

References