Difference between revisions of "Part:BBa K1491029"

 
 
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<partinfo>BBa_K1491029 short</partinfo>
 
<partinfo>BBa_K1491029 short</partinfo>
  
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ER intein T7 RNA polymerase (BBa_K1491025) with constitutive promoter (BBa_J23115) and RBS (BBa_B0034)
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ER intein T7 RNA polymerase (BBa_K1491025) with constitutive promoter (BBa_J23115) and RBS (BBa_B0034). Includes a 6x His tag.
  
 
<!-- Add more about the biology of this part here
 
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Latest revision as of 17:41, 19 September 2014

J23115-B0034-T7 RNA Polymerase with ER Intein

ER intein T7 RNA polymerase (BBa_K1491025) with constitutive promoter (BBa_J23115) and RBS (BBa_B0034). Includes a 6x His tag.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2525
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2950
    Illegal AgeI site found at 2914
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2425