Difference between revisions of "Part:BBa C0600:Design"
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<partinfo>BBa_C0600 SequenceAndFeatures</partinfo> | <partinfo>BBa_C0600 SequenceAndFeatures</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
− | + | This part had XbaI site. The site is cleared by introducing a silent mutation. | |
+ | TCTAGA(at 420) has been changed to CCTAGA. | ||
+ | A codon TAT(Tyr) is changed to TAC(Tyr).Both codons have around 50% abundance in E coli genome. | ||
===Source=== | ===Source=== | ||
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===References=== | ===References=== | ||
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Latest revision as of 07:00, 5 September 2006
Tar
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1282
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 111
Design Notes
This part had XbaI site. The site is cleared by introducing a silent mutation. TCTAGA(at 420) has been changed to CCTAGA. A codon TAT(Tyr) is changed to TAC(Tyr).Both codons have around 50% abundance in E coli genome.