Difference between revisions of "Part:BBa C0600:Design"

(→‎Design Notes)
(→‎Design Notes)
 
Line 9: Line 9:
  
 
This part had XbaI site. The site is cleared by introducing a silent mutation.
 
This part had XbaI site. The site is cleared by introducing a silent mutation.
 +
TCTAGA(at 420) has been changed to CCTAGA.
 +
A codon TAT(Tyr) is changed to TAC(Tyr).Both codons have around 50% abundance in E coli genome.
  
 
===Source===
 
===Source===

Latest revision as of 07:00, 5 September 2006


Tar


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1282
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 111


Design Notes

This part had XbaI site. The site is cleared by introducing a silent mutation. TCTAGA(at 420) has been changed to CCTAGA. A codon TAT(Tyr) is changed to TAC(Tyr).Both codons have around 50% abundance in E coli genome.

Source

References