Difference between revisions of "Part:BBa K1399011"
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Lactose/IPTG inducible promoter with RBS, RFP reporter tagged with LVA-ssrA degradation tag and terminator. | Lactose/IPTG inducible promoter with RBS, RFP reporter tagged with LVA-ssrA degradation tag and terminator. | ||
The tagged RFP is actively degraded within cell, thus provides better temporal resolution of red fluorescence and promoter activity. | The tagged RFP is actively degraded within cell, thus provides better temporal resolution of red fluorescence and promoter activity. | ||
| − | This part can be considered as improved version of [[BBa_J04451]] as it does not contain StuI restriction site coding for additional 'RP' residues between RFP and LVA tag. | + | This part can be considered as improved version of [[Part:BBa_J04451]] as it does not contain StuI restriction site coding for additional 'RP' residues between RFP and LVA tag. |
Revision as of 16:40, 19 September 2014
Plac-RFP(LVA)
Lactose/IPTG inducible promoter with RBS, RFP reporter tagged with LVA-ssrA degradation tag and terminator. The tagged RFP is actively degraded within cell, thus provides better temporal resolution of red fluorescence and promoter activity. This part can be considered as improved version of Part:BBa_J04451 as it does not contain StuI restriction site coding for additional 'RP' residues between RFP and LVA tag.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 781
Illegal AgeI site found at 893 - 1000COMPATIBLE WITH RFC[1000]