Difference between revisions of "Part:BBa K1399010"

(Created page with " == Plac-RFP(AAV) == Lactose/IPTG inducible promoter with RFP reporter tagged with AAV-ssrA degradation tag followed by terminator. The tagged RFP is actively degraded within ce...")
 
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Lactose/IPTG inducible promoter with RFP reporter tagged with AAV-ssrA degradation tag followed by terminator.
 
Lactose/IPTG inducible promoter with RFP reporter tagged with AAV-ssrA degradation tag followed by terminator.
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The tagged RFP is actively degraded within cell, thus it provides better temporal resolution of red fluorescence and promoter activity.  
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The tagged RFP is actively degraded within cell, thus it provides better temporal resolution of red fluorescence and promoter activity.
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Part was assembled using BrickClip assembly (BBF RFC 104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
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[1] Trubitsyna, M., Michlewski, G., Cai, Y., Elfick, A. & French, C. E. PaperClip: rapid multi-part DNA assembly from existing libraries. Nucleic Acids Res. 1–8 (2014). doi:10.1093/nar/gku829
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Revision as of 16:09, 19 September 2014

Plac-RFP(AAV)

Lactose/IPTG inducible promoter with RFP reporter tagged with AAV-ssrA degradation tag followed by terminator. The tagged RFP is actively degraded within cell, thus it provides better temporal resolution of red fluorescence and promoter activity.