Difference between revisions of "Part:BBa K1398003"
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A construct created to degraded TNT and nitroglycerin. | A construct created to degraded TNT and nitroglycerin. | ||
The construct contains the coding sequence for NemA (BBa_K1398002), an enzyme involved in the degradation of toxic compounds for their reuse in nitrogen metabolism. | The construct contains the coding sequence for NemA (BBa_K1398002), an enzyme involved in the degradation of toxic compounds for their reuse in nitrogen metabolism. | ||
| − | The construct also contains a Lactose-inducible promoter (BBa_R0010 ), a strong RBS (BBa_B0034) and a double terminator made up of BBa_B0010 and BBa_B0012 | + | The construct also contains a Lactose-inducible promoter (BBa_R0010 ), a strong RBS (BBa_B0034) and a double terminator made up of BBa_B0010 and BBa_B0012. |
The protein has been codon-optimised for expression in E. coli. | The protein has been codon-optimised for expression in E. coli. | ||
Revision as of 10:05, 19 September 2014
NemA (Inducible Construct)
A construct created to degraded TNT and nitroglycerin. The construct contains the coding sequence for NemA (BBa_K1398002), an enzyme involved in the degradation of toxic compounds for their reuse in nitrogen metabolism. The construct also contains a Lactose-inducible promoter (BBa_R0010 ), a strong RBS (BBa_B0034) and a double terminator made up of BBa_B0010 and BBa_B0012. The protein has been codon-optimised for expression in E. coli.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 746
Illegal AgeI site found at 583 - 1000COMPATIBLE WITH RFC[1000]