Difference between revisions of "Part:BBa K1499500"

 
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<partinfo>BBa_K1499500 short</partinfo>
 
<partinfo>BBa_K1499500 short</partinfo>
  
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This part is the combination of two parts, BBa_I13202 and BBa_T9002. The first part (BBa_I13202) produces a luxI enzyme under the control of a lac repressible promoter. The second part (BBa_T9002) produces luxR, which combines with the luxI to activate the promoter that controls expression of GFP.
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This part is the combination of two parts, BBa_I13202 and BBa_T9002. The first part (BBa_I13202) produces a luxI enzyme which produces an AHL (3OC6HSL). This part is under the control of a lac repressible promoter. The second part (BBa_T9002) produces luxR, which combines with the AHL to activate the luxPR promoter that controls expression of GFP.
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http://2010.igem.org/File:Luxoperon.png
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 20:32, 14 September 2014

quorum sensing machinery that activates GFP expression

This part is the combination of two parts, BBa_I13202 and BBa_T9002. The first part (BBa_I13202) produces a luxI enzyme which produces an AHL (3OC6HSL). This part is under the control of a lac repressible promoter. The second part (BBa_T9002) produces luxR, which combines with the AHL to activate the luxPR promoter that controls expression of GFP.

http://2010.igem.org/File:Luxoperon.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1815
    Illegal BsaI.rc site found at 2543