Difference between revisions of "Part:BBa K1523101"
Ritianjiang (Talk | contribs) |
Ritianjiang (Talk | contribs) |
||
| Line 3: | Line 3: | ||
It can sense p-nitrophenol by Pyear.When PNP exits,it can produce electry in MFC. | It can sense p-nitrophenol by Pyear.When PNP exits,it can produce electry in MFC. | ||
| + | ''' | ||
| + | Abstract''' | ||
| − | |||
Microbial Fuel Cells (MFCs) which can convert contaminants in wastewaters into energy is an ideal approach to solve both pollution problem and energy crisis. However, MFCs is hard to determine the contaminants concentrations which is a major drawback for MFCs applications. We found that the electricity produced by MFCs was somewhat related to the substrates concentrations such as P-nitrophenol (PNP) in anode or Chromium (VI) in cathode. Then a novel MFC system was designed. For anode strain, we’ve cloned a PNP sensor sequence and a riboflavin into Escherichia coli. The recombinants is able to detect PNP and produce riboflavin to boost electrical generation when co-cultured with Shewanella oneidensis. | Microbial Fuel Cells (MFCs) which can convert contaminants in wastewaters into energy is an ideal approach to solve both pollution problem and energy crisis. However, MFCs is hard to determine the contaminants concentrations which is a major drawback for MFCs applications. We found that the electricity produced by MFCs was somewhat related to the substrates concentrations such as P-nitrophenol (PNP) in anode or Chromium (VI) in cathode. Then a novel MFC system was designed. For anode strain, we’ve cloned a PNP sensor sequence and a riboflavin into Escherichia coli. The recombinants is able to detect PNP and produce riboflavin to boost electrical generation when co-cultured with Shewanella oneidensis. | ||
| − | Pathway | + | |
| + | |||
| + | |||
| + | |||
| + | ''' | ||
| + | Pathway''' | ||
[[File:Lzuchina2014-sensor.png]] | [[File:Lzuchina2014-sensor.png]] | ||
Revision as of 02:46, 12 September 2014
PyeaR->Riboflavin synthase
It can sense p-nitrophenol by Pyear.When PNP exits,it can produce electry in MFC. Abstract
Microbial Fuel Cells (MFCs) which can convert contaminants in wastewaters into energy is an ideal approach to solve both pollution problem and energy crisis. However, MFCs is hard to determine the contaminants concentrations which is a major drawback for MFCs applications. We found that the electricity produced by MFCs was somewhat related to the substrates concentrations such as P-nitrophenol (PNP) in anode or Chromium (VI) in cathode. Then a novel MFC system was designed. For anode strain, we’ve cloned a PNP sensor sequence and a riboflavin into Escherichia coli. The recombinants is able to detect PNP and produce riboflavin to boost electrical generation when co-cultured with Shewanella oneidensis.
Pathway
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1243
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]