Difference between revisions of "Part:BBa K1523101"

Revision as of 02:26, 12 September 2014

PyeaR->Riboflavin synthase

It can sense p-nitrophenol by Pyear.When PNP exits,it can produce electry in MFC.

Abstract Microbial Fuel Cells (MFCs) which can convert contaminants in wastewaters into energy is an ideal approach to solve both pollution problem and energy crisis. However, MFCs is hard to determine the contaminants concentrations which is a major drawback for MFCs applications. We found that the electricity produced by MFCs was somewhat related to the substrates concentrations such as P-nitrophenol (PNP) in anode or Chromium (VI) in cathode. Then a novel MFC system was designed. For anode strain, we’ve cloned a PNP sensor sequence and a riboflavin into Escherichia coli. The recombinants is able to detect PNP and produce riboflavin to boost electrical generation when co-cultured with Shewanella oneidensis.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 1243
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K1523101 short</partinfo>
-It can sense p-nitrophenol by Pyear.When PNP exits,it can produce electry in MFC
+It can sense p-nitrophenol by Pyear.When PNP exits,it can produce electry in MFC.
+Abstract
+Microbial Fuel Cells (MFCs) which can convert contaminants in wastewaters into energy is an ideal approach to solve both pollution problem and energy crisis. However, MFCs is hard to determine the contaminants concentrations which is a major drawback for MFCs applications. We found that the electricity produced by MFCs was somewhat related to the substrates concentrations such as P-nitrophenol (PNP) in anode or Chromium (VI) in cathode. Then a novel MFC system was designed. For anode strain, we’ve cloned a PNP sensor sequence and a riboflavin into Escherichia coli. The recombinants is able to detect PNP and produce riboflavin to boost electrical generation when co-cultured with Shewanella oneidensis.
 <!-- Add more about the biology of this part here