Difference between revisions of "Help:Submission"
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<div id="splash-title">Submission Requirements</div> | <div id="splash-title">Submission Requirements</div> | ||
| − | <p>You've <a href="https://parts.igem.org/Help:Adding_Parts">added your part</a> to the Registry, but before you <a href="https://parts.igem.org/cgi/dna_transfer/index.cgi">send a DNA sample</a> to the Registry, | + | <p>You've <a href="https://parts.igem.org/Help:Adding_Parts">added your part</a> to the Registry, but before you <a href="https://parts.igem.org/cgi/dna_transfer/index.cgi">send a DNA sample</a> to the Registry, here's how you prepare your parts for the Registry. Remember, the Registry is built on standard parts!</p> |
<p>If you have questions or issues concerning the requirements, contact <b>hq (at) igem (dot) org</b>.</p> | <p>If you have questions or issues concerning the requirements, contact <b>hq (at) igem (dot) org</b>.</p> | ||
</div> | </div> | ||
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<div class="grid_4"> | <div class="grid_4"> | ||
| − | <div class="box rounded" style="min-height: | + | <div class="box rounded" style="min-height: 400px;"> |
<h3><img src="https://static.igem.org/mediawiki/parts/5/58/List-gear-1.png" style="width: 30px; height: 30px;" /> <a href="https://parts.igem.org/Part:pSB1C3">pSB1C3</a></h3> | <h3><img src="https://static.igem.org/mediawiki/parts/5/58/List-gear-1.png" style="width: 30px; height: 30px;" /> <a href="https://parts.igem.org/Part:pSB1C3">pSB1C3</a></h3> | ||
<ul><b>Parts must be in pSB1C3, <a href="https://parts.igem.org/Help:Shipping">the Registry's standard shipping backbone</a></b> | <ul><b>Parts must be in pSB1C3, <a href="https://parts.igem.org/Help:Shipping">the Registry's standard shipping backbone</a></b> | ||
<li>Your part will be flanked by the <a href="https://parts.igem.org/Help:Prefix-Suffix">BioBrick prefix and suffix</a> on the backbone</li> | <li>Your part will be flanked by the <a href="https://parts.igem.org/Help:Prefix-Suffix">BioBrick prefix and suffix</a> on the backbone</li> | ||
<li>The Registry can test and maintain all parts in the same way</li> | <li>The Registry can test and maintain all parts in the same way</li> | ||
| − | <li>pSB1C3 is a high-copy plasmid, which improves miniprep yields for parts</li> | + | <li>pSB1C3 is a high-copy plasmid, which improves miniprep yields for parts. (If your part is toxic at high copy, use <a href="https://parts.igem.org/Part:pSB3C5">pSB3C5</a>)</li> |
<li>Users can easily move/assemble the part into <a href="https://parts.igem.org/Help:Plasmid_Backbones">another plasmid backbone</a></li> | <li>Users can easily move/assemble the part into <a href="https://parts.igem.org/Help:Plasmid_Backbones">another plasmid backbone</a></li> | ||
<li>You can use the pSB1C3 <a href="https://parts.igem.org/Help:Protocols/Linearized_Plasmid_Backbones">linearized plasmid backbone</a> (in kit or by request) or the pSB1C3 samples in the distribution kit.</li> | <li>You can use the pSB1C3 <a href="https://parts.igem.org/Help:Protocols/Linearized_Plasmid_Backbones">linearized plasmid backbone</a> (in kit or by request) or the pSB1C3 samples in the distribution kit.</li> | ||
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| − | <div class="box rounded" style="min-height: | + | <div class="box rounded" style="min-height: 400px;"> |
<h3><img src="https://static.igem.org/mediawiki/parts/d/d0/List-gear-2.png" style="width: 30px; height: 30px;" /> <a href="https://parts.igem.org/Help:Prefix-Suffix">BioBrick compatible</a></h3> | <h3><img src="https://static.igem.org/mediawiki/parts/d/d0/List-gear-2.png" style="width: 30px; height: 30px;" /> <a href="https://parts.igem.org/Help:Prefix-Suffix">BioBrick compatible</a></h3> | ||
<ul><b>Parts must be <a href="https://parts.igem.org/Help:Assembly_Compatibility">BioBrick (RFC 10) compatible</a> | <ul><b>Parts must be <a href="https://parts.igem.org/Help:Assembly_Compatibility">BioBrick (RFC 10) compatible</a> | ||
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| − | <div class="box rounded" style="min-height: | + | <div class="box rounded" style="min-height: 400px;"> |
<h3><img src="https://static.igem.org/mediawiki/parts/d/dd/List-gear-3.png" style="width: 30px; height: 30px;" /> <a href="https://parts.igem.org/Add_a_Part_to_the_Registry">Documented</a></h3> | <h3><img src="https://static.igem.org/mediawiki/parts/d/dd/List-gear-3.png" style="width: 30px; height: 30px;" /> <a href="https://parts.igem.org/Add_a_Part_to_the_Registry">Documented</a></h3> | ||
<ul><b>Parts shipped to the Registry must be documented on the Registry.</b> | <ul><b>Parts shipped to the Registry must be documented on the Registry.</b> | ||
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<ul>Now that your part meets the Registry's submission requirements, you're ready to <a href="https://parts.igem.org/cgi/dna_transfer/index.cgi">send a DNA sample</a>: | <ul>Now that your part meets the Registry's submission requirements, you're ready to <a href="https://parts.igem.org/cgi/dna_transfer/index.cgi">send a DNA sample</a>: | ||
<li>you'll need to fill out an online submission form</li> | <li>you'll need to fill out an online submission form</li> | ||
| − | <li><a href="https://parts.igem.org/Help:Submission_Form/Formats">prepare the physical shipment</a> (aliquot DNA into tubes or wells)</li> | + | <li><a href="https://parts.igem.org/Help:Submission_Form/Formats">prepare the physical shipment</a> (aliquot DNA into tubes or wells). Avoid leaky tubes! Choose a Registry accepted format!</li> |
<li>ship the DNA to iGEM HQ (include a tracking number)</li> | <li>ship the DNA to iGEM HQ (include a tracking number)</li> | ||
</ul> | </ul> | ||
Revision as of 20:54, 11 September 2014
- Registry Help Pages:
- TOC
- At-a-Glance
- FAQ
Sample submission of parts is no longer a requirement for the iGEM competition.
Requirements
Here's how part samples are sent to the Registry
Why pSB1C3?
Why do samples need to be shipped in pSB1C3?
Detailed Instructions
Instructions on how to fill out the Registry's submission form.
Submission Prep
Registry groups must follow iGEM's submission prep guidelines.
Submission Requirements
You've added your part to the Registry, but before you send a DNA sample to the Registry, here's how you prepare your parts for the Registry. Remember, the Registry is built on standard parts!
If you have questions or issues concerning the requirements, contact hq (at) igem (dot) org.
pSB1C3
- Parts must be in pSB1C3, the Registry's standard shipping backbone
- Your part will be flanked by the BioBrick prefix and suffix on the backbone
- The Registry can test and maintain all parts in the same way
- pSB1C3 is a high-copy plasmid, which improves miniprep yields for parts. (If your part is toxic at high copy, use pSB3C5)
- Users can easily move/assemble the part into another plasmid backbone
- You can use the pSB1C3 linearized plasmid backbone (in kit or by request) or the pSB1C3 samples in the distribution kit.
BioBrick compatible
- Parts must be BioBrick (RFC 10) compatible
- Your part's sequence must not contain the restriction sites of the BioBrick prefix and suffix: EcoRI, XbaI, SpeI, PstI.
- The Registry can test and maintain all parts in the same way
- Your part can be assembled to others with 3A Assembly.
- Users can easily move/assemble the part into another plasmid backbone
- Check if your part is BioBrick compatible by looking at the part's sequence and feature box.
Documented
- Parts shipped to the Registry must be documented on the Registry.
- You must add your part to the Registry; it will then receive a part name (BBa_...)
- Your part's sequence must be entered into the Registry
- Quality over quantity: sending a few well-documented parts is always better than sending several that are not
- For examples check out the collection of well documented parts.
Safety
- iGEM teams must meet the safety requirements
- The Registry accepts parts from Risk Group 1 organisms.
- The Registry may accept parts from Risk Group 2 organisms that have received authorization from the Safety committee
- The Registry will not accept any parts from Risk Group 3 and 4 organisms
- The Registry will not accept any parts from organisms or viruses on The Australia Group's List of Biological Agents for Export Control.
Submission Form
- Now that your part meets the Registry's submission requirements, you're ready to send a DNA sample:
- you'll need to fill out an online submission form
- prepare the physical shipment (aliquot DNA into tubes or wells). Avoid leaky tubes! Choose a Registry accepted format!
- ship the DNA to iGEM HQ (include a tracking number)
Note to iGEM Teams: Your BioBrick Part DNA is due at iGEM HQ by the deadline on the Calendar of Events. Make sure to ship it before the deadline, allowing for transit time.