Difference between revisions of "Part:BBa K1412600"
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<partinfo>BBa_K1412600 short</partinfo> | <partinfo>BBa_K1412600 short</partinfo> | ||
| + | =='''What it is'''== | ||
| − | + | Composite part enables the chemotaxis of the engineering CL-1 could be regulated by AHL and AiiA, which promote the emergence of quorum sensing and oscillation. | |
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| + | =='''What it does'''== | ||
| + | The luxI promoter drives production of the luxI, aiiA, and CheZ genes. LuxI enzymatically produces a small molecule AHL, which can diffuse outside of the cell membrane and into neighboring cells, activating the luxI promoter. AiiA negatively regulates the circuit by acting as an effective protease for AHL.In the beginning,the small colony of individual cells cannot produce enough inducer to activate expression from the luxI promoter. However,once the population reaches a critical density, there is a “burst” of transcription of the luxI promoters, resulting in increased levels of LuxI, AiiA, and CheZ(and the bacteria will move outward). As AiiA accumulates, it begins to degrade AHL, and after a sufficient time, the promoters return to their inactivated state. The production of AiiA is then attenuated, which permits another round of AHL accumulation and another burst of the promoters. | ||
| − | + | ||
| − | == | + | |
| − | + | =='''How to use it in their project'''== | |
| − | + | The emergence of quorum sensing and oscillation will cause the periodic expression of CheZ,which will lead to the growth of bacteria radius present cyclical growth. | |
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| + | =='''Experimental data'''== | ||
Revision as of 04:31, 9 September 2014
Endow the CL-1(E.coli engineered strain) the ability of chemotaxis and quorum sensing
What it is
Composite part enables the chemotaxis of the engineering CL-1 could be regulated by AHL and AiiA, which promote the emergence of quorum sensing and oscillation.
What it does
The luxI promoter drives production of the luxI, aiiA, and CheZ genes. LuxI enzymatically produces a small molecule AHL, which can diffuse outside of the cell membrane and into neighboring cells, activating the luxI promoter. AiiA negatively regulates the circuit by acting as an effective protease for AHL.In the beginning,the small colony of individual cells cannot produce enough inducer to activate expression from the luxI promoter. However,once the population reaches a critical density, there is a “burst” of transcription of the luxI promoters, resulting in increased levels of LuxI, AiiA, and CheZ(and the bacteria will move outward). As AiiA accumulates, it begins to degrade AHL, and after a sufficient time, the promoters return to their inactivated state. The production of AiiA is then attenuated, which permits another round of AHL accumulation and another burst of the promoters.
How to use it in their project
The emergence of quorum sensing and oscillation will cause the periodic expression of CheZ,which will lead to the growth of bacteria radius present cyclical growth.